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Georgios Skretas
Georgios Skretas
National Hellenic Research Foundationhttp://www.eie.gr/nhrf/institutes/ibrb/programmes/enzymology-gr
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Strain engineering for improved expression of recombinant proteins in bacteria
T Makino, G Skretas, G Georgiou
Microbial cell factories 10, 1-10, 2011
2482011
Regulation of protein activity with small‐molecule‐controlled inteins
G Skretas, DW Wood
Protein Science 14 (2), 523-532, 2005
1342005
Efficient production of membrane‐integrated and detergent‐soluble G protein‐coupled receptors in Escherichia coli
AJ Link, G Skretas, EM Strauch, NS Chari, G Georgiou
Protein Science 17 (10), 1857-1863, 2008
882008
Discovery and characterization of a thermostable and highly halotolerant GH5 cellulase from an icelandic hot spring isolate
D Zarafeta, D Kissas, C Sayer, SR Gudbergsdottir, E Ladoukakis, ...
PLoS One 11 (1), e0146454, 2016
762016
Comprehensive engineering of Escherichia coli for enhanced expression of IgG antibodies
T Makino, G Skretas, TH Kang, G Georgiou
Metabolic engineering 13 (2), 241-251, 2011
742011
Metagenomic mining for thermostable esterolytic enzymes uncovers a new family of bacterial esterases
D Zarafeta, D Moschidi, E Ladoukakis, S Gavrilov, ED Chrysina, ...
Scientific reports 6 (1), 38886, 2016
662016
Engineered chimeric enzymes as tools for drug discovery: generating reliable bacterial screens for the detection, discovery, and assessment of estrogen receptor modulators
G Skretas, AK Meligova, C Villalonga-Barber, DJ Mitsiou, MN Alexis, ...
Journal of the American Chemical Society 129 (27), 8443-8457, 2007
662007
A bacterial biosensor of endocrine modulators
G Skretas, DW Wood
Journal of molecular biology 349 (3), 464-474, 2005
552005
Genetic analysis of G protein‐coupled receptor expression in Escherichia coli: inhibitory role of DnaJ on the membrane integration of the human central cannabinoid receptor
G Skretas, G Georgiou
Biotechnology and bioengineering 102 (2), 357-367, 2009
542009
Simple genetic selection protocol for isolation of overexpressed genes that enhance accumulation of membrane-integrated human G protein-coupled receptors in Escherichia coli
G Skretas, G Georgiou
Applied and environmental microbiology 76 (17), 5852-5859, 2010
472010
Expression of active human sialyltransferase ST6GalNAcI in Escherichia coli
G Skretas, S Carroll, S DeFrees, MF Schwartz, KF Johnson, G Georgiou
Microbial cell factories 8, 1-11, 2009
432009
Discovering novel hydrolases from hot environments
R Wohlgemuth, J Littlechild, D Monti, K Schnorr, T van Rossum, B Siebers, ...
Biotechnology advances 36 (8), 2077-2100, 2018
422018
Development of Escherichia coli Strains That Withstand Membrane Protein-Induced Toxicity and Achieve High-Level Recombinant Membrane Protein Production
D Gialama, K Kostelidou, M Michou, DC Delivoria, FN Kolisis, G Skretas
ACS synthetic biology 6 (2), 284-300, 2017
412017
Development of a four-enzyme magnetic nanobiocatalyst for multi-step cascade reactions
A Giannakopoulou, M Patila, K Spyrou, N Chalmpes, D Zarafeta, ...
Catalysts 9 (12), 995, 2019
332019
Multi-copy genes that enhance the yield of mammalian G protein-coupled receptors in Escherichia coli
G Skretas, T Makino, N Varadarajan, M Pogson, G Georgiou
Metabolic engineering 14 (5), 591-602, 2012
322012
ANASTASIA: an automated metagenomic analysis pipeline for novel enzyme discovery exploiting next generation sequencing data
T Koutsandreas, E Ladoukakis, E Pilalis, D Zarafeta, FN Kolisis, G Skretas, ...
Frontiers in genetics 10, 434571, 2019
272019
An integrated bacterial system for the discovery of chemical rescuers of disease-associated protein misfolding
I Matis, DC Delivoria, B Mavroidi, N Papaevgeniou, S Panoutsou, ...
Nature Biomedical Engineering 1 (10), 838-852, 2017
252017
XynDZ5: a new thermostable GH10 xylanase
D Zarafeta, AP Galanopoulou, ME Leni, SI Kaili, MS Chegkazi, ...
Frontiers in microbiology 11, 498087, 2020
232020
Optimization of Recombinant Membrane Protein Production in the Engineered Escherichia coli Strains SuptoxD and SuptoxR
M Michou, C Kapsalis, C Pliotas, G Skretas
ACS Synthetic Biology 8 (7), 1631-1641, 2019
222019
Functional requirements for DjlA-and RraA-mediated enhancement of recombinant membrane protein production in the engineered Escherichia coli strains SuptoxD and SuptoxR
D Gialama, DC Delivoria, M Michou, A Giannakopoulou, G Skretas
Journal of Molecular Biology 429 (12), 1800-1816, 2017
222017
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Artikelen 1–20